Horseradish peroxidase,from one defined source.
Armoracia LLC manufactures unique, high-purity Horseradish Peroxidase (HRP) at its dedicated production facility in Lviv, Ukraine. Armoracia Trade is the official exclusive distributor of this product — a single, traceable supply chain for research laboratories, diagnostic manufacturers, and reagent and kit companies evaluating HRP.
| No. | Property | Value | Remark |
|---|---|---|---|
| 01 | RZ value (A403/A280) | ≥ 3.0 | Reinheitszahl purity ratio |
| 02 | Specific activity | 150–250 U/mg | Substrate-dependent assay |
| 03 | Form | Lyophilized powder | Cold-chain shipped |
| 04 | Storage | 2–8 °C / −20 °C | Short / long term |
| 05 | Source | Armoracia rusticana | Dedicated root cultivation |
Representative specification — request the current Certificate of Analysis for batch-specific values.
From ammonium sulfate suspension to sealed vial
Eight controlled stages, documented per batch at Armoracia LLC, Lviv.
Desalting on Sephadex
The initial ammonium sulfate suspension of the peroxidase enzyme preparation is passed through a Sephadex column — a special gel that separates low-molecular-weight salts, primarily residual ammonium sulfate, from the target protein.
Centrifugation
After desalting, the samples are centrifuged under controlled parameters to remove insoluble material and obtain a clarified solution. The resulting supernatant is used for further chromatographic purification.
Ion-Exchange Chromatography
A key purification step: the solution is slowly passed through an ion-exchange column, where the target enzyme, peroxidase, binds to the resin. Accompanying components and bulk (contaminating) proteins are separated during washing and subsequent elution.
Analytical Control
Each fraction undergoes instrumental analysis: spectrophotometric measurements are used to determine the spectral purity index of the preparation (RZ), calculated as the ratio of optical density at wavelengths of 403 and 275 nm (A403/A275). The measurement results are processed using software and documented in the form of graphs and reports.
Freezing in Cassettes (−50 °C)
The purified solution is dispensed into labeled cassettes and frozen to −50 °C. This step prepares the preparation for lyophilization.
Freeze-Drying (Lyophilization)
After freezing, the preparation undergoes sublimation drying: under reduced pressure, water is removed directly from the solid phase, bypassing the liquid stage. The result is a stable powder with a long shelf life.
Activity Measurement
After freeze-drying, a representative portion of the preparation is taken from each cassette as a sample for quality control. Final control: serial dilutions of the finished preparation are tested for enzymatic activity using a UV spectrophotometer. Based on the measurement results, the activity of the preparation is calculated in the established units, and the obtained values are documented for the corresponding batch. It is this data that is entered into the certificate of quality and accompanying documentation.
Packaging
After quality control has been performed and compliance with the established specifications confirmed, the lyophilized enzyme preparation is weighed and filled into hermetically sealed vials.
ELISA & IHC diagnostics
HRP-conjugate performance for enzyme immunoassay and immunohistochemistry detection systems.
Immunoassay kit manufacturing
Bulk supply for reagent and kit companies formulating HRP-based detection conjugates.
Clinical chemistry
Enzymatic reagent formulations for clinical chemistry analyzers and assay development.
Built on a single, controlled process
Every batch is manufactured and released under one quality system, with documentation available to support your evaluation.
- ISO-certified quality management system
- Certificate of Analysis issued per batch
- Full technical datasheets on request
- Batch-level traceability from raw material to shipment
Armoracia LLC manufacturing facility — Lviv, Ukraine
Certification
Every batch of horseradish peroxidase we ship is manufactured to the highest quality standards and in full compliance with the regulations that govern reagent production, medical-device supply and export from Ukraine. Purification, analytical control and packaging all run under a documented quality-management system, and no lot leaves Lviv until its spectral purity index and enzymatic activity have been measured, recorded and signed off against specification.
That discipline is not a claim — it is paperwork, and we keep all of it. Our certification file covers the company, the quality system, each production batch and every export formality, so your regulatory affairs team can close its file on the first pass rather than the third.
- 01ISO 9001:2015
- 02Utility model patent No. 151216
- 03Test Report No. 23-09-22/1
Please note that we send the set of documents upon request.
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